Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

7.3K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.3K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

9.5K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
9.5K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.7K
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

3.5K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.5K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

10.8K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sticking to Membranes: Structure, Function, and Cellular Roles of the Annexin Family of Ca<sup>2+</sup>- and Membrane-Binding Proteins.

Cold Spring Harbor perspectives in biology·2025
Same author

A Pitstop-2 analog impairs viability of aggressive lung cancer cells by disrupting nuclear pore integrity.

Nanoscale advances·2025
Same author

From Mechanoelectric Conversion to Tissue Regeneration: Translational Progress in Piezoelectric Materials.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Annexin A8 deficiency delays atherosclerosis progression.

Clinical and translational medicine·2025
Same author

Synergistic activity of Pitstop-2 and 1,6-hexanediol in aggressive human lung cancer cells.

Discover nano·2025
Same author

Decoding the regulatory role of ATP synthase inhibitory factor 1 (ATPIF1) in Wallerian degeneration and peripheral nerve regeneration.

Exploration (Beijing, China)·2024

Related Experiment Video

Updated: Mar 9, 2026

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.8K

Annexin A8 promotes VEGF-A driven endothelial cell sprouting.

Nicole Heitzig1, Benjamin F Brinkmann1, Sophia N Koerdt1

  • 1a Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, and Interdisciplinary Clinical Research Center , University of Münster , Münster , Germany.

Cell Adhesion & Migration
|January 7, 2017
PubMed
Summary

Annexin A8 (AnxA8) is crucial for endothelial cell (EC) function in angiogenesis. Its deficiency impairs EC sprouting, migration, and adhesion by disrupting cell surface complex formation, leading to abnormal signaling and vessel growth.

Keywords:
Annexin A8CD63HUVECVEGFR2angiogenesisendothelial cell sproutinginvasionproximity ligation assaytetraspaninβ1 integrin

More Related Videos

In Vitro Model of Coronary Angiogenesis
08:03

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

8.5K
2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

912

Related Experiment Videos

Last Updated: Mar 9, 2026

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.8K
In Vitro Model of Coronary Angiogenesis
08:03

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

8.5K
2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

912

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Angiogenesis, the formation of new blood vessels, is a complex process involving endothelial cell (EC) adhesion, migration, and sprouting.
  • Annexin A8 (AnxA8) is a calcium- and membrane-binding protein implicated in various cellular functions.
  • Vascular Endothelial Growth Factor (VEGF)-A signaling is a key driver of angiogenesis.

Purpose of the Study:

  • To investigate the role of Annexin A8 (AnxA8) in endothelial cell (EC) function during angiogenesis.
  • To elucidate the molecular mechanisms by which AnxA8 influences VEGF-A signaling and EC behavior.

Main Methods:

  • Utilized human umbilical vein endothelial cells (HUVECs) with AnxA8 deficiency.
  • Assessed EC sprouting, migration, and adhesion to extracellular matrix (ECM) proteins.
  • Analyzed cell surface complex formation involving CD63, VEGFR2, and β1 integrin.
  • Examined VEGFR2 internalization, phosphorylation, and focal adhesion kinase (FAK) activation upon VEGF-A stimulation.

Main Results:

  • AnxA8-deficient HUVECs exhibited significantly impaired sprouting, migration, and adhesion.
  • These cells showed defects in forming cell surface complexes of CD63, VEGFR2, and β1 integrin.
  • VEGF-A stimulation led to reduced VEGFR2 internalization and increased VEGFR2 phosphorylation in AnxA8-depleted cells.
  • Altered spatial distribution of phosphorylated focal adhesion kinase (pFAK577) was observed.

Conclusions:

  • AnxA8 plays a critical role in regulating the formation of CD63/VEGFR2/β1 integrin complexes on the EC surface.
  • AnxA8 deficiency results in hyperactivation of the VEGF-A signaling pathway.
  • These molecular alterations lead to severely disturbed VEGF-A-driven angiogenic sprouting.