Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A cryogenic torsion balance using a liquid-cryogen free, ultra-low vibration cryostat.

The Review of scientific instruments·2022
Same author

A low-frequency torsion pendulum with interferometric readout.

The Review of scientific instruments·2021
Same author

A reference-beam autocollimator with nanoradian sensitivity from mHz to kHz and dynamic range of 10(7).

The Review of scientific instruments·2013
Same author

Temporal extent of surface potentials between closely spaced metals.

Physical review letters·2008
Same author

Test of the equivalence principle using a rotating torsion balance.

Physical review letters·2008
Same author

Laboratory test of Newton's second law for small accelerations.

Physical review letters·2007

Related Experiment Video

Updated: Mar 9, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K

Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNT.

A H Laszlo1, I M Derrrington1, J H Gundlach1

  • 1University of Washington, Seattle, WA, United States.

Methods in Enzymology
|January 8, 2017
PubMed
Summary
This summary is machine-generated.

Single-molecule Picometer Resolution Nanopore Tweezers (SPRNT) track enzyme movement on DNA with unprecedented precision. This method reveals enzyme dynamics at the single-nucleotide level, offering new insights into molecular mechanisms.

Keywords:
DNA sequencingEnzyme kineticsForce spectroscopyHigh spatiotemporal resolutionIn vitroMspASingle moleculeSingle-nucleotide resolution

More Related Videos

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.2K
Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

14.1K

Related Experiment Videos

Last Updated: Mar 9, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.2K
Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

14.1K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biotechnology

Background:

  • Nanopore technology is advancing single-molecule studies of enzymes.
  • Existing methods lack the spatiotemporal precision to observe enzyme dynamics in real-time.

Purpose of the Study:

  • To introduce the Single-molecule Picometer Resolution Nanopore Tweezers (SPRNT) technique.
  • To demonstrate SPRNT's capability in analyzing enzyme activity at high resolution.

Main Methods:

  • Loading an enzyme onto DNA/RNA and threading it into a protein nanopore (MspA).
  • Measuring ion current changes through the nanopore as DNA translocates, controlled by enzyme activity.
  • Utilizing picometer spatial resolution and millisecond temporal resolution for real-time analysis.

Main Results:

  • SPRNT achieved 40pm spatial resolution and millisecond timescale measurements.
  • Observed distinct ATP-dependent and ATP-independent substates during helicase Hel308 activity.
  • Provided real-time DNA sequence information within the enzyme complex.

Conclusions:

  • SPRNT offers a low-cost, high-precision single-molecule method for studying enzyme dynamics.
  • The technique enables the observation of previously unresolvable enzyme movements in real-time.
  • SPRNT significantly advances the field of enzyme mechanism studies.