Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pathological extracellular matrix changes in decellularized normal appearing gray matter and subpial multiple sclerosis lesions.

iScience·2026

Same author

Assessment of the bacteria-derived metabolite TMAO as a potential factor contributing to the pathophysiology of delirium in elderly patients with hip fracture.

Brain, behavior, and immunity·2026

Same author

Tim-3-targeted vaccines overcome tumor immunosuppression and reduce cDC1 dependence to elicit potent anti-tumor immunity.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

B cells maintain the homeostasis of splenic marginal zone antigen-presenting cells to promote the antiviral CD8<sup>+</sup> T-cell response.

Cellular & molecular immunology·2026

Same author

Stable serum peptidoglycan fragment levels do not support leaky gut in the acute phase or at one month following "mild" traumatic brain injury: A preliminary study.

Brain, behavior, & immunity - health·2026

Same author

Spinal fluid antibody profile against bacteria demonstrates intrathecal antibody production in demyelinating diseases.

Journal of molecular medicine (Berlin, Germany)·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 9, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models.

Jon D Laman¹, Susanne M Kooistra², Björn E Clausen³

¹Section Medical Physiology, Department of Neuroscience, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, NL-9713 AV, Groningen, The Netherlands. j.d.laman@umcg.nl.

Methods in Molecular Biology (Clifton, N.J.)

|January 8, 2017

Summary

This summary is machine-generated.

Reproducibility in animal models for inflammatory diseases is crucial for advancing biomedical research and developing new therapies. This study highlights common pitfalls in preclinical animal research to improve model reliability.

Keywords:

Animal models Immunity Inflammation Lymphocytes Microbiome Microbiota Myeloid cells Publication bias Randomization Repeatability Replication Reproducibility Research assessment Robustness Statistics Transgenic artifact

More Related Videos

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Published on: May 16, 2016

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Related Experiment Videos

Last Updated: Mar 9, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Published on: May 16, 2016

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Area of Science:

Biomedical Research
Immunology
Neuroscience

Background:

Ethical concerns and rising costs impact animal research.
Debates exist regarding the reproducibility and human relevance of animal models for inflammatory diseases.
Despite advancements in alternatives (the three R's: replace, reduce, refine), in vivo models remain critical for understanding complex inflammatory interactions.

Purpose of the Study:

To summarize potential pitfalls in preclinical animal research.
To provide resources and background reading for improving the reproducibility of animal inflammation models.
To support scientific advancement and the development of novel therapeutic interventions.

Main Methods:

Review and summarization of common challenges in preclinical animal studies.
Identification of critical factors affecting the reproducibility of animal inflammation models.
Compilation of resources and background literature for researchers.

Main Results:

Preclinical animal research faces numerous potential pitfalls that can compromise results.
Improving the reproducibility of animal models is essential for scientific progress.
Understanding complex immune, nervous, and tissue interactions in inflammation relies on reliable in vivo data.

Conclusions:

Addressing pitfalls in animal research is vital for enhancing reproducibility.
Reliable animal models are indispensable for advancing our understanding of human inflammatory diseases.
This work serves as a prelude to practical protocols for improving animal model research.