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Toxicity Testing in Animals

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...

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Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Published on: October 20, 2020

Protein-based nanotoxicology assessment strategy.

Marlene Pedersen Elnegaard¹, Markus List², Helle Christiansen¹

¹Lundbeckfonden Center of Excellence in Nanomedicine NanoCAN, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Nanomedicine : Nanotechnology, Biology, and Medicine

|January 9, 2017

Summary

Standardized in vitro assays for nanoparticle toxicity are needed. Chip-based protein detection offers a complementary method to assess nanodrug toxicity, improving hit selection for further analysis.

Keywords:

Cell viability Nanotoxicology Protein array RNAi

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Last Updated: Mar 9, 2026

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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Area of Science:

Biotechnology
Nanotoxicology
Cellular Biology

Background:

Standardized in vitro assays are crucial for nanoparticle toxicity assessment.
Reducing in vivo experiments is a key goal in nanomaterial research.
Existing assays may not capture the full spectrum of cellular effects.

Purpose of the Study:

To evaluate chip-based protein detection as a method for assessing nanodrug toxicity.
To compare protein detection with traditional cytotoxicity assays.
To identify cellular effects of nanodrug mimics using a differential screening approach.

Main Methods:

Transiently transfected siRNAs were used as nanodrug mimics in MCF7 and MCF12A cells.
Cell viability was measured as a cytotoxicity endpoint.
Protein expression of glyceraldehyde-3-phosphate dehydrogenase, transferrin receptor, and Ki67 was analyzed.
Chip-based protein detection was employed for sensitive analysis.

Main Results:

Chip-based protein detection effectively assessed nanodrug toxicity.
Combined analysis of cell lethality and protein expression revealed cellular effects missed by single methods.
The study successfully mimicked a differential screen using nanodrugs.

Conclusions:

Chip-based protein detection is a suitable method to complement traditional assays for nanoparticle toxicity.
This approach can improve the selection of primary hits for subsequent in vivo validation.
Integrated analysis of cytotoxicity and protein expression provides a more comprehensive understanding of cellular responses to nanomaterials.