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Mechanisms of Membrane Domain Formation

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Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
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Labeling membrane domains in dividing echinoderm eggs.

M Bennett¹, J Meaders¹, S Ruvolo¹

¹Boston College, Chestnut Hill, MA, United States.

Methods in Cell Biology

|January 10, 2017

Summary

This summary is machine-generated.

Sea urchins are valuable models for cell biology. This chapter details methods for live-cell imaging of membrane dynamics during sea urchin embryo cytokinesis.

Keywords:

Cytokinesis Lipid rafts Live-cell imaging Membrane labeling Methods Sea urchins

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Area of Science:

Cell Biology
Developmental Biology
Biophysics

Background:

Sea urchins are established model organisms for cell biology and developmental research.
Their large, manageable eggs and synchronized embryonic divisions facilitate experimental manipulation.
Previous research has utilized sea urchins to study fertilization, mitosis, and chromosome separation.

Purpose of the Study:

To present methods for studying membrane dynamics during cytokinesis in sea urchin embryos.
To focus on live-cell imaging techniques for visualizing membrane domains.

Main Methods:

Live-cell imaging
Microinjection and manipulation of sea urchin embryos
Focus on membrane domain visualization during cytokinesis

Main Results:

Detailed methods for live-cell imaging of membrane dynamics are provided.
The chapter facilitates the study of membrane domain behavior during cell division.
Established protocols for working with sea urchin embryos are leveraged.

Conclusions:

Sea urchin embryos offer a robust system for investigating membrane dynamics in cell division.
The presented methods enable detailed observation of membrane domain behavior during cytokinesis.
This work contributes to understanding fundamental processes in cell biology and development.