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Related Concept Videos

DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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Related Experiment Video

Updated: Mar 9, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

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G-quadruplexes unfolding by RHAU helicase.

Nassima Meriem Gueddouda1, Oscar Mendoza1, Dennis Gomez2

  • 1Univ. Bordeaux, INSERM U1212, CNRS UMR 5320, ARNA Laboratory, IECB, F-33607 Pessac, France.

Biochimica Et Biophysica Acta. General Subjects
|January 10, 2017
PubMed
Summary

Researchers developed a fast, inexpensive assay to study interactions between G-quadruplexes (G4) and the RHAU helicase. This new method monitors G4 unfolding by RHAU in DNA and RNA, aiding research into genome stability and cancer risk.

Keywords:
DHX36G-quadruplexG4 ligandG4R1HelicaseHelicase assayRHAU

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Related Experiment Videos

Last Updated: Mar 9, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • G-quadruplexes (G4) are non-canonical nucleic acid structures crucial in genomic regulation.
  • Proteins like helicases, including RHAU, are involved in resolving G4 structures.
  • Dysfunctional helicases are linked to genome instability and cancer.

Purpose of the Study:

  • To develop a high-throughput, fluorescence-based assay for studying G4/RHAU interactions.
  • To enable parallel monitoring of RHAU's unfolding activity on DNA and RNA quadruplexes.
  • To facilitate screening for optimal conditions for RHAU helicase activity.

Main Methods:

  • A novel, high-throughput fluorescence-based assay was established.
  • The assay measures the unfolding of G-quadruplex structures by the RHAU helicase.
  • DNA and RNA quadruplexes were analyzed in parallel.

Main Results:

  • The assay is reliable, inexpensive, and fast for studying G4/RHAU interactions.
  • It allows for the parallel assessment of RHAU's unfolding properties on both DNA and RNA G4s.
  • The method facilitates the screening of optimal conditions for RHAU activity.

Conclusions:

  • The developed assay provides a robust tool for investigating G-quadruplex resolution by helicases.
  • This method can advance the understanding of RHAU's role in G4 metabolism and its implications for genome stability.
  • The assay supports research into potential therapeutic targets related to G4 structures and helicase function.