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Sustained Nitric Oxide Release from a Tertiary S-Nitrosothiol-based Polyphosphazene Coating.

Alec Lutzke1, Jesus B Tapia1, Megan J Neufeld1

  • 1Department of Chemistry, ‡School of Biomedical Engineering, and §Department of Chemical & Biological Engineering, Colorado State University , Fort Collins, Colorado 80523, United States.

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Summary

This study introduces a new biodegradable polymer coating that releases therapeutic nitric oxide (NO) for up to two weeks. The advanced material offers improved NO storage and release for biomedical applications.

Keywords:
S-nitrosothiolsbiodegradationbiomaterialsnitric oxidepolyphosphazenes

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Biomedical Engineering

Background:

  • Nitric oxide (NO) is a vital signaling molecule with antithrombotic, antibacterial, and wound-healing properties.
  • Existing NO-releasing polymers often suffer from limited NO storage and short-term release, hindering their biomedical applications.
  • Development of advanced materials with enhanced NO release function is crucial for biointerfacial applications.

Purpose of the Study:

  • To develop a novel NO-releasing bioerodible coating using a polyphosphazene-based polymer.
  • To evaluate the NO release kinetics, storage capacity, and degradation profile of the developed coating.
  • To assess the potential of this new material for therapeutic NO delivery in biomedical applications.

Main Methods:

  • Synthesis of poly[bis(3-mercapto-3-methylbut-1-yl glycinyl)phosphazene] (POP-Gly-MMB).
  • Conversion of the thiolated polymer to an S-nitrosothiol (RSNO) derivative (POP-Gly-MMB-NO) for NO formation.
  • Measurement of NO release flux using chemiluminescence; determination of NO storage capacity and degradation products via TOF-MS.

Main Results:

  • The POP-Gly-MMB-NO coating released NO at a physiologically relevant flux (6.5-0.090 nmol·min-1·cm-2) for up to 2 weeks in PBS at 37 °C.
  • The coating demonstrated a high NO storage capacity of 0.89 ± 0.09 mmol·g-1.
  • Hydrolytic degradation over 6 weeks resulted in 14% mass loss, yielding glycine, MMB, and related esters.

Conclusions:

  • The developed POP-Gly-MMB-NO coating offers sustained, long-term therapeutic nitric oxide release.
  • Its high NO storage capacity and controlled degradation profile make it a promising candidate for biomedical applications.
  • This material represents a significant advancement over existing NO-releasing polymers for tissue engineering and medical devices.