Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.6K
The Nucleus01:32

The Nucleus

107.6K
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
107.6K
The Nucleus01:25

The Nucleus

8.2K
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
8.2K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Chromatin Packaging02:21

Chromatin Packaging

22.7K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Confined migration induces non-lethal DNA damage in developing neurons.

Nature·2026
Same author

Boundary constraints can determine pattern emergence.

Development (Cambridge, England)·2026
Same author

Flexible high-resolution ECM micropatterning.

Nature protocols·2026
Same author

Hedgehog-driven adaxial cell constriction patterns slow muscle fate and somite boundary remodeling in the presomitic mesoderm.

Cell reports·2026
Same author

A new tunable 3D alveolospheres model from human alveolar epithelial type 2 cells (AEC2) with reduced heterogeneity for studying cigarette smoke extract exposure.

Respiratory research·2026
Same author

Identification of optimal fluorophores for use in the <i>Drosophila</i> embryo.

Molecular biology of the cell·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K

3D Protein Dynamics in the Cell Nucleus.

Anand P Singh1, Rémi Galland2, Megan L Finch-Edmondson3

  • 1Mechanobiology Institute, National University of Singapore, Singapore.

Biophysical Journal
|January 12, 2017
PubMed
Summary
This summary is machine-generated.

We developed a novel microscopy technique to measure 3D nuclear protein dynamics. This method reveals spatial differences in how key proteins like Polymerase II move within live cells.

More Related Videos

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.6K
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
05:47

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Published on: July 29, 2018

17.2K

Related Experiment Videos

Last Updated: Mar 9, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.6K
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
05:47

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Published on: July 29, 2018

17.2K

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • The 3D nuclear architecture influences gene expression and protein behavior.
  • Understanding nuclear protein dynamics is crucial but challenging.
  • Current methods lack the resolution to capture 3D nuclear protein movement.

Purpose of the Study:

  • To develop and validate a novel method for quantitatively measuring 3D nuclear protein dynamics.
  • To investigate spatial heterogeneity in protein diffusion within the cell nucleus.
  • To provide insights into the movement of specific proteins like Polymerase II and human-Yes-associated protein.

Main Methods:

  • Combined single-objective light-sheet microscopy with photoconvertible proteins.
  • Utilized fluorescence correlation microscopy to analyze protein diffusion.
  • Acquired over 3400 autocorrelation functions across the nucleus with minimal photobleaching.

Main Results:

  • Successfully quantified 3D protein dynamics in the nucleus.
  • Demonstrated spatial heterogeneity in Polymerase II diffusion in live U2OS cells.
  • Measured diffusion dynamics of human-Yes-associated protein in gastric cancer cells.

Conclusions:

  • The novel microscopy technique enables reliable measurement of 3D nuclear protein dynamics.
  • Revealed spatial variations in protein movement within the nucleus.
  • Provides a powerful tool for studying nuclear function and gene regulation.