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Related Concept Videos

Gap Junctions01:27

Gap Junctions

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The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...
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Exon Recombination02:32

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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Related Experiment Video

Updated: Mar 9, 2026

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
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Virus Innexins induce alterations in insect cell and tissue function.

Daniel K Hasegawa1, Stephanie L Erickson1, Bradley M Hersh2

  • 1Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA.

Journal of Insect Physiology
|January 13, 2017
PubMed
Summary
This summary is machine-generated.

Polydnavirus vinnexins, viral genes mimicking insect innexins, cause gene-specific host developmental changes. These findings aid understanding virus-host interactions and insect innexin functions.

Keywords:
Gap junctionHemocytic encapsulationInnexinPolydnavirusVinnexin

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Area of Science:

  • Virology
  • Insect Molecular Biology
  • Cellular Physiology

Background:

  • Polydnaviruses are dsDNA viruses that manipulate caterpillar hosts.
  • Vinnexins are polydnavirus genes homologous to insect innexins, involved in gap junctions.
  • Innexin roles in insect immunity are poorly understood.

Purpose of the Study:

  • To investigate the physiological effects of vinnexin gene expression in host cells and organisms.
  • To explore the potential of vinnexins in understanding insect innexin function.
  • To characterize gene-specific roles of vinnexins in host manipulation.

Main Methods:

  • Ectopic vinnexin expression in lepidopteran cell cultures.
  • Ectopic vinnexin and innexin2 expression in Drosophila melanogaster models (cell culture, whole organism).
  • Assessment of cell morphology, gap junction activity (dye transfer), embryonic lethality, and developmental arrest.

Main Results:

  • Vinnexin expression altered cell morphology in culture; effects on gap junction activity were minor.
  • Ectopic vinnexinG or Dm-inx2 expression caused embryonic lethality in Drosophila.
  • VinnexinG expression in larval gut led to pupal developmental arrest.

Conclusions:

  • Vinnexins exhibit gene-specific roles in manipulating host physiology.
  • Drosophila is a suitable model for studying polydnavirus genes like vinnexins.
  • Vinnexin genes can be valuable tools for characterizing insect innexin functions.