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Updated: Aug 6, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Ti plasmid-controlled chromosome transfer in Agrobacterium tumefaciens
Y Dessaux1, A Petit, J G Ellis
1Groupe de Recherche sur les Interactions entre Microorganismes et Plantes, Institut de Microbiologie, Faculté des Sciences, Orsay, France.
Abstract:
In octopine-type A. tumefaciens R10, transfer of chromosomal arginine degradation genes (arc genes) was observed under conditions in which Ti plasmid transfer took place. However, transconjugants that had acquired the arc genes but not the Ti plasmid were recovered. During this process, several other chromosomal genes, such as genes encoding phage resistances or genes complementing a galactose utilization mutation or a glycine-serine auxotrophy, were transferred from strain R10 to the recipient.
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