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Related Experiment Video

Updated: Mar 8, 2026

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Error analysis in newborn screening: can quotients support the absolute values?

Borros Arneth1, Martin Hintz2

  • 1Institute of Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics, Hospital of the Universities of Giessen and Marburg (UKGM), Justus Liebig University Giessen, Feulgenstr. 12, 35392, Giessen, Germany. borros.arneth@klinchemie.med.uni-giessen.de.

Analytical and Bioanalytical Chemistry
|January 14, 2017
PubMed
Summary

Newborn screening using tandem mass spectrometry shows derived quotients are more precise than absolute concentrations. These analytical ratios support accurate diagnosis by improving measurement reliability.

Keywords:
Measurement errorMetabolic patternMetabolismNewborn screeningTandem mass spectrometry

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Mass Spectrometry

Background:

  • Newborn screening utilizes tandem mass spectrometry to detect various amino acids and fatty acids.
  • This method measures absolute concentrations and derived quotients for diagnostic markers.

Purpose of the Study:

  • To determine and compare the precision of absolute analyte concentrations and derived quotients in newborn screening.
  • To evaluate the analytical accuracy and diagnostic support of these parameters.

Main Methods:

  • Practical determination of measurement errors for absolute concentrations and ratios.
  • Application of Gaussian error calculation theory.
  • Comparative analysis of measurement errors between absolute values and quotients.

Main Results:

  • Derived quotients demonstrated significantly higher analytical accuracy (e.g., Phe/Tyr quotient CV = 5.1%) compared to absolute concentrations (mean CV = 12%).
  • The Fisher quotient also showed high precision (CV = 5.6%).

Conclusions:

  • Derived quotients are analytically accurate and offer superior precision over absolute concentrations in tandem mass spectrometry.
  • These precise ratios can analytically support absolute values, enhancing diagnostic accuracy in newborn screening.