Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thiaheptapyrrin and Thiatetrapyrrin Armed p-Phenylene-Bridged Norrole as NIR-II Dyes: Photothermal Behavior Effectively Enhanced by Protonation and Deprotonation.

Chemistry, an Asian journal·2026
Same author

Interaction of Polymer of Intrinsic Microporosity PIM‑1 with Explosive Analytes at the Molecular Level: Combined Experiment and Computational Modeling.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Structure of Fluoride Anion Aqueous Solution Derived from X-ray Spectroscopy.

The journal of physical chemistry. B·2026
Same author

Single-molecule electrical characterization of photoinduced aggregation evolution.

Nature communications·2026
Same author

Synergistic Dual-Passivation via Indium Doping and Zwitterionic Ligands for Efficient Pure-Blue Perovskite Light-Emitting Diodes.

ACS applied materials & interfaces·2026
Same author

Visualizing Senescent-Normal Cell Boundaries Through Environment-Dependent Bidirectional Luminescent Contrast.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Mar 8, 2026

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

16.0K

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications.

Haichun Liu1, Muthu K G Jayakumar2, Kai Huang2

  • 1Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, 117583 Singapore, Singapore. biezy@nus.edu.sg and Division of Theoretical Chemistry and Biology, Royal Institute of Technology, S-10691 Stockholm, Sweden.

Nanoscale
|January 14, 2017
PubMed
Summary
This summary is machine-generated.

Lanthanide-doped upconversion nanoparticles (UCNPs) can now be encoded using luminescence kinetics, specifically the phase angle, significantly boosting multiplexing capabilities. This new dimension expands UCNP applications in bioimaging, data storage, and anti-counterfeiting.

More Related Videos

Triplet Fusion Upconversion Nanocapsule Synthesis
08:36

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

3.0K
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.9K

Related Experiment Videos

Last Updated: Mar 8, 2026

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

16.0K
Triplet Fusion Upconversion Nanocapsule Synthesis
08:36

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

3.0K
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.9K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Optics

Background:

  • Lanthanide-doped upconversion nanoparticles (UCNPs) are valuable for multiplexing due to their optical properties.
  • Existing UCNP encoding methods include emission color, intensity ratios, spatial distribution, and luminescence lifetime.

Purpose of the Study:

  • To develop a novel optical encoding dimension for upconversion nanomaterials using luminescence kinetics.
  • To explore the phase angle of upconversion luminescence as a new encoding parameter.

Main Methods:

  • Theoretical derivation of phase angle dependence on luminescence rise and decay times.
  • Experimental development of methods to control upconversion luminescence kinetics.
  • Manipulation of rise and decay times independently or dependently.

Main Results:

  • Demonstrated that phase angle is governed by luminescence kinetics (rise and decay times).
  • Achieved a large phase-angle space for generating unique codes within the same color channel.
  • Successfully manipulated luminescence kinetics to create distinct codes.

Conclusions:

  • Introduced luminescence kinetics (phase angle) as a new multiplexing dimension for UCNPs.
  • Significantly extended the multiplexing capacity of UCNPs.
  • Opened new avenues for UCNP applications in bioassays, imaging, anti-counterfeiting, and data storage.