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Updated: May 6, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Visualizing Senescent-Normal Cell Boundaries Through Environment-Dependent Bidirectional Luminescent Contrast.

Pan-Xin Ge1,2,3, Yuqing Hu3,4, Lu-Lu Sun2

  • 1School of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.

Angewandte Chemie (International Ed. in English)
|May 5, 2026
PubMed
Summary
This summary is machine-generated.

New luminescent probes precisely distinguish senescent from normal cells by modulating signals based on the cellular environment. This breakthrough aids surgical decisions by clarifying tissue boundaries, preventing recurrence and preserving tissue integrity.

Keywords:
bidirectional photomodulationglyco‐persulfurated arenesluminescent probesenescent cells

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Cellular Biology

Background:

  • Senescent tissues lack clear cellular boundaries, complicating surgical removal and risking recurrence or tissue damage.
  • Current probes cannot differentiate between normal and senescent cells, leading to ambiguous signals.
  • Precise delineation of senescent-normal cell boundaries is critical for effective surgical interventions.

Purpose of the Study:

  • To develop novel luminescent probes for accurate discrimination between senescent and normal cells.
  • To design probes with environment-dependent bidirectional signal modulation for dynamic contrast.
  • To address the ambiguity in surgical decision-making for senescent tissues.

Main Methods:

  • Constructed luminescent probes by modifying hydrophilic β-galactose groups onto hydrophobic hydroxyl-hexathiobenzene.
  • Engineered probes for environment-dependent bidirectional photomodulation.
  • Utilized β-galactosidase (β-Gal)-triggered deglycosylation and photoexcitation-induced aggregation/reorganization.

Main Results:

  • Probes remain non-emissive in normal cells but become emissive upon photoexcitation-induced aggregation (Direction 1).
  • In senescent cells, β-Gal triggers deglycosylation, generating emissive aggregation-induced emission products, which can be subsequently down-regulated (Direction 2).
  • Achieved dynamic contrast between senescent and normal cells using a single probe system.

Conclusions:

  • Developed a new paradigm for resolving biological ambiguity at the cellular scale.
  • The bidirectional photomodulation probes enable precise delineation of senescent-normal cell boundaries.
  • This technology offers significant potential for improved surgical guidance in senescent tissue management.