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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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MicroRNA-432 targeting E2F3 and P55PIK inhibits myogenesis through PI3K/AKT/mTOR signaling pathway

Meilin Ma1, Xiangming Wang1, Xiaochang Chen1

  • 1a Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi , China.

RNA Biology
|January 14, 2017
PubMed

Insights

MicroRNA-432 (miRNA-432) inhibits skeletal muscle growth by targeting E2F3 and P55PIK. This microRNA (miRNA) impacts cell cycle and PI3K/AKT/mTOR signaling, crucial for myogenesis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Skeletal muscle development and metabolism rely on intricate gene regulation.
  • MicroRNAs (miRNAs) are key post-transcriptional regulators involved in various biological processes, including myogenesis.
  • Specific miRNAs, like miR-432, show differential expression during muscle development.

Purpose of the Study:

  • To investigate the role of miR-432 in skeletal muscle development.
  • To identify the molecular targets and mechanisms through which miR-432 regulates myogenesis.

Main Methods:

  • miRNA expression profiling in pig muscle tissues.
  • Gain-of-function studies to assess miR-432's impact on myoblast proliferation and differentiation.
  • Target gene identification and validation (E2F3, P55PIK).
  • Analysis of downstream signaling pathways (PI3K/AKT/mTOR).

Main Results:

  • miR-432 expression is higher in young piglets compared to adult pigs.
  • Overexpression of miR-432 inhibits myoblast proliferation and differentiation.
  • miR-432 directly targets and downregulates E2F3, affecting cell cycle and myogenic genes.
  • miR-432 also targets P55PIK, inhibiting the PI3K/AKT/mTOR pathway during differentiation.
  • Insulin treatment can rescue the inhibitory effect of miR-432 on the PI3K/AKT/mTOR pathway.

Conclusions:

  • miR-432 acts as a significant inhibitor of myogenesis.
  • Its mechanism involves targeting both E2F3 and P55PIK, thereby regulating cell cycle and the PI3K/AKT/mTOR signaling pathway.
  • These findings highlight miR-432 as a potential regulator in skeletal muscle growth and metabolism.
Keywords:
E2F3P55PIKPI3K/Akt/mTOR pathwaymicroRNA-432myogenesis

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