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Genomic Transformation of the Picoeukaryote Ostreococcus tauri
Published on: July 13, 2012
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A development and an improvement of selectable markers in Pleurotus ostreatus transformation
Yukari Matsunaga1, Megumi Ando1, Kosuke Izumitsu1
1School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
Journal of Microbiological Methods
|January 15, 2017
Abstract:
Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.
