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Related Experiment Videos

Imaging proteins at the single-molecule level.

Jean-Nicolas Longchamp1, Stephan Rauschenbach2, Sabine Abb2

  • 1Physics Department of the University of Zurich, CH-8057 Zurich, Switzerland; longchamp@physik.uzh.ch k.kern@fkf.mpg.de hwfink@physik.uzh.ch.

Proceedings of the National Academy of Sciences of the United States of America
|January 15, 2017
PubMed
Summary

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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This summary is machine-generated.

Low-energy electron holography images individual proteins and protein complexes without radiation damage. This technique reveals distinct protein conformations at subnanometer resolution, advancing structural biology and biophysics.

Area of Science:

  • * Structural biology
  • * Biophysics
  • * Molecular nanotechnology

Background:

  • * Imaging single proteins is crucial for understanding their structure and function.
  • * Revealing distinct protein conformations at the individual level is a significant challenge.
  • * Existing methods often involve averaging or can cause radiation damage.

Purpose of the Study:

  • * To demonstrate the capability of low-energy electron holography for imaging individual proteins and protein complexes.
  • * To achieve subnanometer resolution imaging without inducing radiation damage.
  • * To visualize distinct protein conformations and structures at the single-molecule level.

Main Methods:

  • * Preparation of individual proteins and complexes on freestanding graphene using soft-landing electrospray ion beam deposition.
Keywords:
low-energy electron holographymicroscopypreparative mass spectrometrysingle protein imagingstructural biology

Related Experiment Videos

  • * Utilizing low-energy electron holography for imaging.
  • * Employing chemical- and conformational-specific selection during sample preparation.

    • Main Results:

    • * Successful imaging of individual proteins (cytochrome C, BSA) and protein complexes (hemoglobin) was achieved.
    • * Subnanometer resolution images were obtained without radiation damage.
    • * The technique captured images of individual molecules, not averaged structures.

    Conclusions:

    • * Low-energy electron holography is a powerful, non-damaging technique for imaging single proteins and complexes.
    • * This method enables the visualization of distinct protein conformations and structures.
    • * The findings have significant implications for structural biology, biophysics, and molecular nanotechnology.