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Targeting Argonaute to chromatin.

Jered M Wendte1, Craig S Pikaard1,2,3

  • 1Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.

Genes & Development
|January 15, 2017
PubMed
Summary

Small interfering RNAs (siRNAs) guide gene silencing complexes in eukaryotes. Shimada et al. found that in fission yeast, these complexes target DNA exclusively through interactions with newly forming RNA transcripts.

Keywords:
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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Small interfering RNAs (siRNAs) bound to Argonaute proteins mediate transcriptional gene silencing in eukaryotes by guiding chromatin modifiers.
  • siRNAs are known to interact with longer RNAs at target loci, but direct base-pairing with DNA has been hypothesized.

Purpose of the Study:

  • To investigate the mechanism by which siRNA-Argonaute complexes are recruited to target loci in fission yeast.
  • To differentiate between siRNA-DNA and siRNA-RNA interactions in transcriptional gene silencing.

Main Methods:

  • Experimental investigation of siRNA-Argonaute complex recruitment in fission yeast.
  • Analysis of interactions between siRNAs, Argonaute proteins, nascent transcripts, and DNA at target loci.

Main Results:

  • Evidence indicates that siRNA-Argonaute complexes are recruited to target loci exclusively through interactions with nascent RNA transcripts.
  • The study provides strong support against the hypothesis of direct siRNA-DNA base-pairing for recruitment.

Conclusions:

  • Fission yeast transcriptional gene silencing relies on siRNA-Argonaute complexes interacting with nascent transcripts, not directly with DNA.
  • This finding clarifies the molecular mechanism of RNA-guided gene silencing in this model organism.