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Related Concept Videos

Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Proteomics: Technologies and Their Applications.

Bilal Aslam1, Madiha Basit1, Muhammad Atif Nisar1

  • 1Department of Microbiology, Government College University, Faisalabad, Pakistan.

Journal of Chromatographic Science
|January 15, 2017
PubMed
Summary
This summary is machine-generated.

Proteomics identifies and quantifies proteins, aiding research in disease and diagnostics. Despite its complexity and cost, advancements in proteomics technologies offer valuable insights into biological systems.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Proteomics analyzes the complete set of proteins within a biological system.
  • It complements genomics and transcriptomics for a comprehensive understanding of biological functions.
  • Proteomics applications span diagnostics, vaccine development, and disease mechanism elucidation.

Purpose of the Study:

  • To review various proteomics approaches.
  • To highlight recent developments in proteomics technologies.
  • To discuss the applications of proteomics in research and analysis.

Main Methods:

  • Mass spectrometry (MS) techniques, including LC-MS-MS and MALDI-TOF/TOF, are central to current proteomics.
  • Analysis involves identifying and quantifying proteins from complex biological samples.
  • Bioinformatics tools and databases are essential for data interpretation.

Main Results:

  • Proteomics enables the detection of diagnostic markers and vaccine candidates.
  • It aids in understanding disease pathogenesis and altered protein expression patterns.
  • The dynamic nature of the proteome presents challenges in analysis.

Conclusions:

  • Proteomics is a powerful tool for biological research and medical diagnostics.
  • Technological advancements are expanding the scope and accessibility of proteomics.
  • Addressing cost and complexity barriers is crucial for wider adoption, especially in developing regions.