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Multi-response optimization of Artemia hatching process using split-split-plot design based response surface

V V Arun1, Neelam Saharan1, V Ramasubramanian1

  • 1ICAR-Central Institute of Fisheries Education, Mumbai, India.

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|January 17, 2017
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A new Box-Behnken Design (BBD) and Split-Split Plot Design (SSPD) method optimizes animal egg hatching. This approach efficiently determined optimal conditions for brine shrimp Artemia hatching, achieving 96.8% success.

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Area of Science:

  • Experimental Design
  • Aquaculture Optimization
  • Biological Process Engineering

Background:

  • Optimizing animal egg hatching requires understanding complex environmental factors.
  • Traditional multi-factorial experiments can be resource-intensive.
  • Efficient experimental designs are crucial for aquaculture and biological research.

Purpose of the Study:

  • To introduce and validate a novel combined experimental design method, BBD-SSPD.
  • To determine the optimal conditions for brine shrimp (Artemia) cyst hatching.
  • To assess the impact of photoperiod, temperature, and salinity on hatching success and time.

Main Methods:

  • Combined Box-Behnken Design (BBD) with Split-Split Plot Design (SSPD) for efficient experimental run selection.
  • Utilized Derringer's desirability function for simultaneous optimization of multiple responses (hatching percentage and time).
  • Investigated three levels each of photoperiod, temperature, and salinity for Artemia cysts.

Main Results:

  • Photoperiod, temperature, and their interactions significantly influenced hatching percentage and time.
  • Temperature-salinity interaction also significantly affected hatching percentage.
  • Optimal conditions identified: 23h photoperiod, 29°C temperature, 28 ppt salinity, yielding 96.8% hatching in 18.94h.

Conclusions:

  • The BBD-SSPD method provides an economical and efficient approach for multi-factorial experiments.
  • This novel design is effective for optimizing the hatching process of animal eggs, specifically demonstrated with Artemia.
  • The study validates the reliability of the BBD-SSPD method through verification experiments.