Synthesis of Silver Nanoparticles using Azadirachta indica and Cinnamomum verum Extract and Its Antibacterial Action
Neena Chandran1, Sindhu Ramesh1, Sabir Muliyar2
1Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, India.
Objective:
To evaluate the antibacterial efficacy of neem and cinnamon extract-mediated AgNPs against Enterococcus faecalis and assess their cytotoxicity.
Materials And Methods:
Neem (Azadirachta indica) and cinnamon (Cinnamomum verum) extracts were employed to reduce silver nitrate, synthesizing AgNPs, with visual changes confirming the reaction. Antibacterial properties were assessed using Mueller-Hinton agar for zone of inhibition (ZOI) and minimal inhibitory concentration (MIC) assays. Cytotoxicity was evaluated on nauplii models.
Results:
Neem-based AgNPs exhibited significantly larger ZOIs compared to cinnamon-based AgNPs against E. faecalis (P < 0.05). MIC assays demonstrated both bacteriostatic and bactericidal effects. Cytotoxicity results showed acceptable safety profiles for both AgNP types.
Conclusion:
Neem and cinnamon extracts are viable agents for green AgNP synthesis, with neem-based AgNPs showing promising antibacterial efficacy against E. faecalis.
