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Updated: Mar 8, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Hemispherical platinum : silver core : shell nanoparticles for miRNA detection.

Elaine Spain¹, Kellie Adamson², Mohammad Elshahawy²

¹School of Chemical Sciences, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland. Robert.forster@dcu.ie Elaine.spain2@mail.dcu.ie and Biomedical Diagnostics Institute, Research & Engineering Building, Dublin City University, Glasnevin, Dublin 9, Ireland.

|January 17, 2017

Summary

This summary is machine-generated.

This study presents a novel method for detecting microRNA (miRNA) using platinum-silver core-shell nanoparticles. This technique enables highly sensitive detection of miRNA associated with neuroblastoma without amplification.

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Area of Science:

Nanotechnology
Biosensing
Electrochemistry

Background:

Defects in self-assembled monolayers on gold serve as nucleation sites for platinum nanoparticle electrodeposition.
Platinum nanoparticles are decorated with silver to form core-shell structures, enabling regioselective functionalization.

Purpose of the Study:

To develop a highly sensitive and specific method for microRNA detection.
To create novel platinum-silver core-shell nanoparticles for biosensing applications.
To investigate the potential of these nanoparticles for early cancer diagnosis.

Main Methods:

Electrodeposition of platinum nanoparticles on gold surfaces with defect sites.
Decoration of platinum nanoparticles with silver to form core-shell structures.
Immobilization of thiolated probe miRNA onto the silver surface.
Detachment of functionalized nanoparticles using a current jump.
Hybridization assay with capture miRNA on an electrode and target miRNA.
Electrocatalytic detection of hybridized nanoparticles via hydrogen peroxide reduction.
Investigation of nanoparticle properties within gold microcavity arrays.

Main Results:

Achieved linear calibration plots for microRNA detection from 1 aM to 1 μM.
Demonstrated detection of attomolar (aM) concentrations of microRNA without chemical amplification.
Successfully immobilized regioselectively modified nanoparticles within gold microcavity arrays.
Investigated the Raman properties of immobilized nanoparticles.

Conclusions:

Developed a sensitive and regioselective nanoparticle-based platform for microRNA detection.
The method shows promise for early detection of neuroblastoma-associated microRNA (miR-132).
The nanoparticle system can be utilized for both solution-based detection and immobilization in microcavity arrays.