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Updated: Mar 8, 2026

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Single Upconversion Nanoparticle-Bacterium Cotrapping for Single-Bacterium Labeling and Analysis.

Hongbao Xin^1,2,3, Yuchao Li⁴, Dekang Xu⁴

¹Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Nanophotonics, Jinan University, Guangzhou, 511443, China.

Small (Weinheim an Der Bergstrasse, Germany)

|January 17, 2017

Summary

This summary is machine-generated.

This study introduces a novel optical method for precisely labeling and analyzing individual pathogenic bacteria using upconversion nanoparticles (UCNPs). This technique enables real-time, single-bacterium detection and analysis, crucial for understanding cellular heterogeneity.

Keywords:

optical connecting optical cotrapping single-bacterium analysis single-bacterium labeling

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Area of Science:

Biophotonics
Nanotechnology
Microbiology

Background:

Accurate detection and analysis of pathogenic bacteria are vital for diagnosing infections and guiding antibiotic treatment.
Analyzing bacteria at the single-cell level is challenging yet essential for understanding cellular heterogeneity and environmental responses.

Purpose of the Study:

To develop an optical strategy for precise single-bacterium labeling and analysis.
To demonstrate the cotrapping of single upconversion nanoparticles (UCNPs) with individual bacteria.

Main Methods:

Optical trapping of a single upconversion nanoparticle (UCNP) with an average size of ≈120 nm.
Simultaneous optical trapping and labeling of both ends of a single bacterium with single UCNPs emitting green light.
Real-time detection of signals from bacteria of varying sizes for individual analysis.

Main Results:

Successful cotrapping and labeling of individual bacteria with single UCNPs.
Demonstrated flexible manipulation of labeled bacteria for targeted analysis.
Real-time detection and analysis of signals from individual bacteria were achieved.

Conclusions:

The cotrapping method offers a new approach for labeling, detecting, and analyzing single pathogenic bacteria.
This technique allows for real-time, single-particle, and single-bacterium level analysis.
Enables detailed study of bacterial heterogeneity and responses.