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Updated: Mar 8, 2026
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
Published on: January 15, 2015
Design and evaluation of a stearylated multicomponent peptide-siRNA nanocomplex for efficient cellular siRNA delivery
Yu Wan1,2, Peter M Moyle2, Pei Z Gn2
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
Aim:
To develop a new synthetic peptide-based nanoparticulate siRNA delivery system.
Materials & Methods:
DEN-K(GALA)-TAT-K(STR) was generated by incorporating stearic acid into a multicomponent peptide (DEN-K(GALA)-TAT), containing a cationic poly-L-lysine dendron, an endosome-disrupting peptide GALA and a cell-penetrating peptide TAT(48-60). Its physicochemical characteristics, size, toxicity, cellular uptake and gene knockdown activity of the peptide/siRNA complexes were studied.
Results:
DEN-K(GALA)-TAT-K(STR) exhibited a pH-responsive behavior, which assists with endosomal escape. When siRNA was delivered by DEN-K(GALA)-TAT-K(STR), it showed a significantly enhanced cellular uptake, compared with the nonlipidic peptide. This system also displayed enhanced knockdown efficiency and reduced cytotoxicity over the widely used delivery system branched 25-kDa polyethyleneimine.
Conclusion:
Our stearylated multicomponent delivery system has great potential as an efficient siRNA delivery vector.
