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Myosin-driven transport network in plants.

Elizabeth G Kurth1, Valera V Peremyslov1, Hannah L Turner1

  • 1Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331.

Proceedings of the National Academy of Sciences of the United States of America
|January 19, 2017
PubMed
Summary
This summary is machine-generated.

We uncovered the myosin XI transport network in Arabidopsis, revealing key roles for myosin-binding proteins (MadA, MadB) in plant cell trafficking and development. This network

Keywords:
adaptorscytoplasmic streamingmyosinsnuclear transportreceptors

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Area of Science:

  • Plant Cell Biology
  • Molecular Motors
  • Biochemistry

Background:

  • Myosin motors are crucial for intracellular transport in eukaryotic cells.
  • Myosin XI family members are implicated in endomembrane trafficking and cytoplasmic streaming in plants.
  • Understanding the myosin XI network is key to deciphering plant cell organization and development.

Purpose of the Study:

  • To elucidate the structure and function of the myosin XI-driven transport network in Arabidopsis.
  • To identify novel components and regulatory mechanisms of myosin XI-mediated transport.
  • To investigate the evolutionary history and functional diversification of myosin XI and its associated proteins.

Main Methods:

  • Protein-protein interaction assays
  • Subcellular localization studies
  • Gene knockout analyses in Arabidopsis
  • Bioinformatics and phylogenetic analyses

Main Results:

  • Identified two novel families of myosin-binding proteins, MadA and MadB, acting as adaptors.
  • Demonstrated myosin XI-driven nucleocytoplasmic transport for MadA1.
  • Showed MadB1-4 are essential for polarized root hair growth, phenocopying myosins.
  • Revealed congruent evolutionary histories and expansions of myosin XI, MyoB, MadA, and MadB families since green algae.

Conclusions:

  • The myosin XI transport network is highly connected, featuring generalist and specialist components.
  • Myosin XI-driven transport is involved in diverse cellular processes, including nucleocytoplasmic trafficking and polarized growth.
  • The expansion and increased complexity of this network likely contributed to the land colonization by flowering plants.