Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.4K
2.4K
The Replisome03:01

The Replisome

39.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
39.1K
The Replisome03:01

The Replisome

10.8K
10.8K
Replication in Prokaryotes02:35

Replication in Prokaryotes

100.5K
Overview
100.5K
Replication in Prokaryotes01:32

Replication in Prokaryotes

28.7K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
28.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the reproducibility of the social and behavioural sciences.

Nature·2026
Same author

Investigating the replicability of the social and behavioural sciences.

Nature·2026
Same author

Investigating the analytical robustness of the social and behavioural sciences.

Nature·2026
Same author

A framework for assessing the trustworthiness of scientific research findings.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Science becomes trustworthy by constantly questioning itself.

PLoS biology·2025
Same author

How can we make sound replication decisions?

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Mar 8, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

Making sense of replications.

Brian A Nosek1,2, Timothy M Errington1

  • 1Center for Open Science, Charlottesville, United States.

Elife
|January 20, 2017
PubMed
Summary
This summary is machine-generated.

The Reproducibility Project: Cancer Biology found that pre-clinical cancer research shows room for improvement. These findings highlight the need for enhanced reproducibility in scientific studies.

Keywords:
Reproducibility Project: Cancer Biologycancer biologymetasciencemethodologyopen sciencereplicationreproducibility

More Related Videos

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.4K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.9K

Related Experiment Videos

Last Updated: Mar 8, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K
Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.4K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.9K

Area of Science:

  • Oncology
  • Translational Research
  • Scientific Methodology

Background:

  • Reproducibility is crucial for scientific advancement.
  • Variability in pre-clinical research can hinder translation to clinical practice.
  • The Reproducibility Project: Cancer Biology aims to assess and improve research reliability.

Purpose of the Study:

  • To evaluate the reproducibility of pre-clinical cancer biology studies.
  • To identify factors impacting the reliability of experimental results.
  • To provide recommendations for enhancing reproducibility in the field.

Main Methods:

  • Replication of selected pre-clinical cancer biology experiments.
  • Standardized protocols and data collection.
  • Statistical analysis of results from original and replicated studies.

Main Results:

  • Initial findings indicate variability in the outcomes of replicated experiments.
  • Specific experimental procedures and reporting practices were identified as potential sources of irreproducibility.
  • The project identified areas where methodological improvements could enhance reliability.

Conclusions:

  • There is a clear need to improve reproducibility in pre-clinical cancer research.
  • Implementing standardized methods and transparent reporting is essential.
  • Further efforts are required to ensure the reliability of cancer research findings.