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Related Experiment Video

Updated: Mar 8, 2026

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

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Published on: January 25, 2019

Compact diode laser source for multiphoton biological imaging.

Robert D Niederriter¹, Baris N Ozbay², Gregory L Futia²

¹Department of Physics, University of Colorado, 390 UCB, Boulder, CO 80309-0390, USA.

Biomedical Optics Express

|January 20, 2017

Summary

This summary is machine-generated.

We developed a compact, tunable diode laser for multiphoton microscopy. It achieves deep tissue imaging of biological samples, enabling enhanced visualization of cellular structures.

Keywords:

(140.3538) Lasers, pulsed (140.5960) Semiconductor lasers (180.2520) Fluorescence microscopy (180.4315) Nonlinear microscopy

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Highly-Multiplexed Tissue Imaging with Raman Dyes

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Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Area of Science:

Biophotonics and Imaging
Laser Technology
Microscopy

Background:

Multiphoton microscopy (MPM) requires specialized laser sources for deep tissue imaging.
Existing sources can be bulky or lack tunability, limiting their application in biological studies.
Fluorescent proteins are crucial for labeling biological structures in MPM.

Purpose of the Study:

To develop a compact, tunable pulsed diode laser source for multiphoton microscopy.
To optimize the laser for common fluorescent markers used in biological imaging.
To assess the laser's performance, including depth penetration in biological tissues.

Main Methods:

Designed and constructed a fiber-coupled pulsed diode laser system.
Operated the laser at a 976 nm center wavelength, suitable for common fluorescent proteins.
Electrically tuned the repetition rate from 66.67 kHz to 10 MHz, with 2.3 ps pulses and >1 kW peak power.

Main Results:

Demonstrated a compact and scalable laser package.
Achieved >600 μm depth penetration in brain tissue, limited primarily by laser power.
Laser parameters are optimized for efficient two-photon excitation of common biological fluorophores.

Conclusions:

The developed diode laser is a viable, compact source for multiphoton microscopy.
Its tunable repetition rate and wavelength offer flexibility for various biological imaging applications.
The laser enables deep tissue imaging, advancing the study of biological samples.