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Updated: Mar 8, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
Chemical and structural effects of base modifications in messenger RNA
Emily M Harcourt1, Anna M Kietrys2, Eric T Kool2
1Department of Chemistry, Wellesley College, Wellesley, Massachusetts 02481, USA.
Abstract:
A growing number of nucleobase modifications in messenger RNA have been revealed through advances in detection and RNA sequencing. Although some of the biochemical pathways that involve modified bases have been identified, research into the world of RNA modification - the epitranscriptome - is still in an early phase. A variety of chemical tools are being used to characterize base modifications, and the structural effects of known base modifications on RNA pairing, thermodynamics and folding are being determined in relation to their putative biological roles.
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