Related Experiment Video

Updated: Mar 8, 2026

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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Prostaglandin E2 receptor 4 mediates renal cell carcinoma intravasation and metastasis

Yushan Zhang1, Hamsa Thayele Purayil1, Joseph B Black1

  • 1Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.

Cancer Letters
|January 21, 2017
PubMed

Insights

Targeting prostaglandin E2 receptor 4 (EP4) can reduce metastatic renal cell carcinoma (RCC) spread. Inhibiting EP4 lowers cancer cell invasion and metastasis by downregulating CD24 and P-selectin, offering new therapeutic avenues.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metastasis Research

Background:

  • Metastatic renal cell carcinoma (RCC) presents limited treatment options.
  • Prostaglandin E2 (PGE2) receptor 4 (EP4) role in RCC metastasis is not well understood.

Purpose of the Study:

  • To investigate the impact of EP4 on RCC metastasis.
  • To elucidate the molecular mechanisms by which EP4 influences cancer cell invasion.

Main Methods:

  • Investigated EP4 knockdown effects on RCC cell lines (ACHN, SN12C) in vivo (xenografts) and in vitro (transendothelial migration assays).
  • Utilized chick chorioallantoic membrane (CAM) assay to assess tumor intravasation.
  • Analyzed gene expression changes, focusing on CD24 and P-selectin interactions.

Main Results:

  • EP4 knockdown reduced RCC metastasis in mice without affecting tumor growth.
  • EP4 signaling blockade and EP4 knockdown inhibited tumor cell intravasation and transendothelial migration (TEM).
  • EP4 knockdown decreased CD24 expression, a P-selectin ligand, and P-selectin inhibition blocked EP4-mediated TEM and RCC intravasation.

Conclusions:

  • EP4 inhibition attenuates RCC intravasation and metastasis by downregulating CD24.
  • P-selectin plays a crucial role in RCC tumor intravasation.
  • EP4, CD24, and P-selectin represent potential therapeutic targets for advanced RCC treatment.
Keywords:
CD24EP4IntravasationMetastasisRenal cell carcinoma

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