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Flow cytometry dataset for cells collected from touched surfaces.

Ye Jin Kwon1, Cristina E Stanciu1, M Katherine Philpott1

  • 1Department of Forensic Science, Virginia Commonwealth University, Richmond, USA.

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Forensic touch DNA mixtures are challenging. This study explored optical signatures of epidermal cells to help separate DNA from different individuals, aiming for clearer genetic profiles.

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epithelial cellflow cytometryforensic sciencetouch mixtures

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Area of Science:

  • Forensic Science
  • Biotechnology
  • Genetics

Background:

  • Trace DNA mixtures from "touch" evidence pose significant interpretation challenges in forensic analysis.
  • Current methods struggle to resolve complex mixtures, impacting the reliability of genetic profiling.

Purpose of the Study:

  • To investigate optical signatures of epidermal cells for differentiating contributors in touch DNA samples.
  • To assess the potential for physical separation of mixture components based on optical properties.

Main Methods:

  • Antibody hybridization surveys using Human Leukocyte Antigen (HLA) and Cytokeratin (CK) probes.
  • Analysis of optical properties: forward scatter (FSC), side scatter (SSC), and Allophycocyanin (APC) fluorescence.
  • Testing on "touch" samples from multiple contributors deposited over several days to evaluate variability.

Main Results:

  • Characterization of optical signatures (FSC, SSC, APC fluorescence) of deposited epidermal cells.
  • Demonstration of inter- and intra-contributor variability in these optical properties.
  • Data supporting the feasibility of distinguishing cell populations from different individuals.

Conclusions:

  • Optical signatures offer a potential method for distinguishing epidermal cell populations from different contributors.
  • This approach could enable physical separation of mixture components, leading to simplified genetic profiles.
  • Further development may improve the interpretation of complex forensic DNA evidence.