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Published on: June 23, 2023
Headspace Fourier transform infrared spectroscopy for the differentiation of Pandanus species
Kim Christopher C Aganda1, Maribel G Nonato1, Fortunato Sevilla1
1Graduate School, and Research Center for Natural and Applied Sciences, University of Santo Tomas, España Blvd., Sampaloc, Manila, 1015 Philippines.
Abstract:
Headspace Fourier Transform Infrared Spectroscopy (HS-FTIR) in tandem with chemometrics was applied to differentiate several species of the genus Pandanus. The headspace was generated from each Pandanus sample after incubation in a tightly sealed sample chamber. The resulting FTIR spectra of the headspace samples were found to be almost similar, but the application of principal component analysis (PCA) effectively differentiated the species. The unique spectral features for some samples were highlighted in the second-derivative FTIR spectra. A higher variance was exhibited in the PCA bi-plot of the 2nd derivative spectral data. The principal components differentiated not only the species, but also the cultivars or varieties, which formed distinct but proximate clusters. The manner of clustering obtained in this study resembled the behavior reported in a Random Amplified Polymorphic DNA analysis conducted on the Pandanus samples. The results demonstrate the potential of headspace FTIR spectroscopy as a simple, rapid, non-destructive, and relatively inexpensive method to discriminate between plant species and varieties.
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