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A label-free electrochemical immunosensor based on a new polymer containing aldehyde and ferrocene groups
Xiangyang Zhang1, Youming Shen2, Youyu Zhang3
1Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China.
Talanta
|January 22, 2017
Summary
A novel polymer film functionalized with aldehyde and ferrocene groups was synthesized. This polymer CAF film enables sensitive and specific label-free electrochemical detection of human IgG.
Area of Science:
- Polymer Chemistry
- Electrochemistry
- Biosensing
Background:
- Development of advanced materials for biosensing applications is crucial.
- Functional polymers offer versatile platforms for sensor fabrication.
Purpose of the Study:
- To synthesize a novel polymer containing aldehyde and ferrocene groups.
- To fabricate a label-free electrochemical immunosensor using the synthesized polymer.
- To evaluate the immunosensor's performance for human IgG detection.
Main Methods:
- Grafting 4-hydroxybenzaldehyde and ferrocenecarboxylhydrazide onto linear poly(vinylbenzyl chloride).
- Fabrication of a polymer film (polymer CAF) as a support matrix.
- Development of a label-free electrochemical immunosensor.
- Detection of human IgG using electrochemical methods.
Main Results:
- A well-defined polymer with aldehyde and ferrocene groups was successfully synthesized.
- The polymer CAF film served as an effective matrix for a label-free electrochemical immunosensor.
- The immunosensor demonstrated high sensitivity and repeatability for human IgG detection.
- A linear concentration range of 0.1 to 20 ng/mL and a detection limit of 0.07 ng/mL were achieved.
- The immunosensor exhibited high specificity, distinguishing human IgG from interfering proteins.
Conclusions:
- The developed polymer synthesis strategy is novel and convenient.
- The polymer CAF-based electrochemical immunosensor is a promising tool for sensitive and specific human IgG detection.
- This approach offers a new support matrix for label-free electrochemical immunosensor fabrication.
