Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization of cell lines persistently infected with infectious pancreatic necrosis virus.

The Journal of general virology·2026

Same author

Evolutionary Conservation, Expansion and Diversification of Interferon stimulated genes (ISG) in Vertebrates: insights from fish.

Biomedical journal·2026

Same author

Erratum: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026

Same author

Development and Validation of a Multiplex RT-PCR Assay for the Simultaneous Detection of Three Major Viral Pathogens in Salmonids: IHNV, ISAV and SAV.

Journal of fish diseases·2026

Same author

N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026

Same author

Toll-like Receptor 3 is Required for Sensing Extracellular Double Stranded RNA in Salmonid Cells.

Marine biotechnology (New York, N.Y.)·2026

Search research articles

Related Experiment Video

Updated: Mar 8, 2026

Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

Engineered cell lines for fish health research.

Bertrand Collet¹, Catherine Collins¹, Katherine Lester¹

¹Marine Scotland, Marine Laboratory, Aberdeen, UK.

Developmental and Comparative Immunology

|January 22, 2017

Summary

Genetically engineered fish cell lines offer promising tools for studying fish diseases and immunology. These cell-based systems aid in understanding fish immune responses and pathogen interactions, advancing aquaculture research.

Keywords:

Cell line Gene silencing Genetically engineered Over-expression Reporter

More Related Videos

Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

Published on: August 17, 2018

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Published on: January 20, 2023

Related Experiment Videos

Last Updated: Mar 8, 2026

Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

Published on: August 17, 2018

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Published on: January 20, 2023

Area of Science:

Aquaculture
Fish Immunology
Genomics

Background:

Global fish farming expansion drives need for advanced fish disease and immunology research.
Genomic information and molecular technologies are rapidly advancing fish research.
Lack of reagents like monoclonal antibodies hinders functional analysis of fish molecules.

Purpose of the Study:

To review available permanently genetically engineered fish cell lines.
To explore their use as cell-based reporter systems or expression platforms.
To investigate fish immune and pathogen gene interactions and functions.

Main Methods:

Overview of existing genetically engineered fish cell lines.
Discussion of cell-based reporter systems.
Exploration of expression platforms for immune or pathogen genes.

Main Results:

Genetically engineered fish cell lines serve as valuable tools for functional genomics.
These systems facilitate the study of molecular interactions in fish diseases.
Reporter systems enable investigation of cellular interactions and gene functions.

Conclusions:

Genetically engineered fish cell lines are crucial for advancing fish disease and immunology research.
These platforms offer significant advantages for studying functional genomics in fish.
Technical challenges in development are acknowledged but surmountable.