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Down-regulation of ASIC1 suppressed gastric cancer via inhibiting autophagy

Qiong Zhang1, Shiwu Wu1, Jinhai Zhu2

  • 1Department of Pathology, Bengbu Medical College, Bengbu 233000, China; Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.

Gene
|January 24, 2017
PubMed

Insights

Targeting acid-sensing ion channels (ASICs) may treat gastric cancer. Downregulating ASIC1 inhibits cancer growth by reducing autophagy, offering a potential therapeutic strategy for gastric cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Autophagy plays a role in cancer cell survival and is a therapeutic target.
  • Acid-sensing ion channels (ASICs) are known to activate autophagy.
  • The role of ASICs in gastric cancer progression via autophagy remains unclear.

Purpose of the Study:

  • To investigate the role of ASICs, specifically ASIC1, in gastric cancer.
  • To determine if ASICs regulate gastric cancer through autophagy.
  • To explore ASIC1 as a potential therapeutic target for gastric cancer.

Main Methods:

  • RNA sequencing to identify differentially expressed genes in gastric cancer.
  • Real-time PCR to confirm expression of ASIC1 and autophagy-related gene 5 (ATG5).
  • Cell proliferation assays (CCK-8) and xenograft models in mice to assess tumor growth and survival rates.

Main Results:

  • Autophagy-related genes, including ASIC1 and ATG5, are upregulated in gastric cancer tissues.
  • Knockdown of ASIC1 or ATG5 inhibits gastric cancer cell growth (SGC-7901).
  • ASIC1 knockdown reduces tumor volume and improves survival rates in a murine model, mediated by decreased autophagy.

Conclusions:

  • ASIC1 expression is elevated in gastric cancer and promotes tumor growth.
  • ASIC1 regulates autophagy in gastric cancer cells.
  • Downregulation of ASIC1 inhibits gastric cancer progression by suppressing autophagy, suggesting ASIC1 as a potential therapeutic target.
Keywords:
ASIC1ATG5AutophagyGastric cancerKnockdown

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