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Simultaneous determination of lactate, pyruvate, and ascorbate in microdialysis samples from rat brain, blood, fat,
A Hallström1, A Carlsson, L Hillered
1Department of Pharmacology, Karolinska Institutet, Carnegie Medicin AB, Stockholm, Sweden.
Journal of Pharmacological Methods
|September 1, 1989
Abstract:
An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.