Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determination of Crystal Structures

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

$X-ray Diffraction of Biological Samples$ 01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrafast X-ray Pump-Probe Investigation of the Formation Dynamics of SiV Centers in Diamond.

Journal of the American Chemical Society·2026

Same author

Pyrite (001) Interface Chemistry is Controlled by a Sulfoxy Termination.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Enhanced Stability of Halide Perovskite by Strengthening Hydrogen Bonds.

JACS Au·2026

Same author

Pressure-Induced 18 K Superconductivity and Two Superconducting Phases in CuIr_{2}S_{4}.

Physical review letters·2026

Same author

High-Pressure Synthesis of Antimony Nitride Sb<sub>3</sub>N<sub>5</sub> and Polynitride Sb<sub>2</sub>N<sub>8</sub> Featuring Single-Bonded N<sub>8</sub> <sup>10-</sup> Chains.

Angewandte Chemie (International ed. in English)·2026

Same author

X-ray-diffraction and electrical-transport imaging of superconducting superhydride (La,Y)H<sub>10</sub>.

Nature communications·2025

Search research articles

Related Experiment Video

Updated: Mar 8, 2026

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

High Pressure Single Crystal Diffraction at PX^2.

Dongzhou Zhang¹, Przemyslaw K Dera², Peter J Eng³

¹Hawai'i Institute of Geophysics and Planetology, University of Hawai'i at Manoa; dzhang@hawaii.edu.

Journal of Visualized Experiments : Jove

|January 25, 2017

Summary

This summary is machine-generated.

This study details single crystal X-ray diffraction experiments using a diamond anvil cell (DAC) for high-pressure mineral physics. Researchers successfully determined the crystal structure of omphacite at 0.35 GPa.

More Related Videos

$Synthesis and Microdiffraction at Extreme Pressures and Temperatures$

Synthesis and Microdiffraction at Extreme Pressures and Temperatures

Published on: October 7, 2013

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Related Experiment Videos

Last Updated: Mar 8, 2026

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

$Synthesis and Microdiffraction at Extreme Pressures and Temperatures$

Synthesis and Microdiffraction at Extreme Pressures and Temperatures

Published on: October 7, 2013

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Area of Science:

Mineral Physics
Crystallography
High-Pressure Science

Background:

Single crystal X-ray diffraction is crucial for determining mineral structures under extreme conditions.
Diamond Anvil Cells (DACs) enable the generation of high pressures necessary for these studies.
Standardized procedures are needed for reliable high-pressure crystallographic experiments.

Purpose of the Study:

To outline detailed procedures for single crystal X-ray diffraction experiments using a DAC.
To present a case study on collecting and analyzing diffraction data from omphacite at high pressure.
To establish a methodology for high-pressure crystallographic studies at the GSECARS 13-BM-C beamline.

Main Methods:

Utilized BX-90 type DACs with conical anvils and noble gas pressure media for hydrostatic conditions.
Employed the MARCCD area detector calibrated with LaB6 for data collection.
Analyzed diffraction data using ATREX and indexed/refined structures with RSV software.

Main Results:

Successfully collected single crystal diffraction data from an omphacite sample at 0.35 GPa.
Determined the crystal structure to be monoclinic with P2/n space group.
Obtained precise lattice parameters: a = 9.496 ±0.006 Å, b = 8.761 ±0.004 Å, c = 5.248 ±0.001 Å, β = 105.06 ±0.03º.

Conclusions:

The described procedures are effective for high-pressure single crystal X-ray diffraction.
The study provides a refined crystal structure of omphacite at 0.35 GPa.
This work contributes to the Partnership for Extreme Xtallography (PX^2) project.