Coupling Activity-Based Detection, Target Amplification, Colorimetric and Fluorometric Signal Amplification, for
Xiaolong Sun1, James F Reuther1, Scott T Phillips2
1Department of Chemistry, The University of Texas at Austin, Austin, TX, 78712, USA.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 25, 2017
Summary
A new method detects G-class nerve agents by amplifying fluoride release. This sensitive chemosensing protocol uses oximates and autoinductive reactions for colorimetric and fluorescent detection.
Area of Science:
- Analytical Chemistry
- Chemical Sensors
- Organic Chemistry
Background:
- G-class nerve agents readily produce fluoride upon reaction with nucleophiles.
- Existing detection methods may lack sensitivity or selectivity for these agents.
Purpose of the Study:
- To develop a sensitive and selective chemosensing protocol for G-class nerve agents.
- To utilize autoinductive fluoride amplification for enhanced optical detection.
Main Methods:
- Designed a protocol pairing oximates for fluoride generation with autoinductive amplification.
- Employed diisopropyl fluorophosphate (DFP) as a nerve agent surrogate and benzaldoxime as the nucleophile.
- Utilized colorimetric and fluorescent reporters for quantification.
Main Results:
- Achieved low micromolar limits of detection for fluoride derived from DFP.
- Demonstrated successful amplification of fluoride concentration via an autoinductive reaction.
- Generated a yellow reporter molecule and a fluorescent readout with three fluorophores per fluoride.
Conclusions:
- The developed protocol enables selective, sensitive, and quantitative chemosensing of G-class nerve agents.
- Successfully merged multiple complex reactions for effective fluoride detection.
- Offers a promising approach for optical quantification of nerve agents.
