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Updated: Mar 8, 2026

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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
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Chemical probing of thiotetronate bio-assembly
Judith Havemann1, Marie E Yurkovich2, Robert Jenkins1
1Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
Abstract:
Chemical 'chain termination' probes were utilised for the investigation of thiotetronate antibiotic biosynthesis in the filamentous bacteria Lentzea sp. and Streptomyces thiolactonus NRRL 15439. The use of these tools led to the capture of biosynthetic intermediates involved in the thiotetronate polyketide backbone assembly, providing first insights into substrate specificity and in vivo intermediate processing by unusual iterative synthases.

