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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Mar 8, 2026

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Published on: February 21, 2013

Mechanical Characterization of Microengineered Epithelial Cysts by Using Atomic Force Microscopy.

Yusheng Shen¹, Dongshi Guan², Daniela Serien³

¹Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong.

Biophysical Journal

|January 26, 2017

Summary

This summary is machine-generated.

This study reveals how the mechanical properties of epithelial cysts are influenced by their internal lumen. Understanding these mechanics is key to comprehending tissue physiology and disease.

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Last Updated: Mar 8, 2026

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

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Published on: February 21, 2013

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

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Published on: August 28, 2011

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

Area of Science:

Biophysics
Cell Biology
Tissue Engineering

Background:

Epithelial tissues form one-cell-thick monolayers with lumens, crucial for organ development and physiology.
Tissue function relies on biochemical and mechanical regulation, with mechanical aspects being less understood.
Polarized epithelial cysts are simple models for studying lumen-enclosing tissues.

Purpose of the Study:

To develop an in vitro platform for characterizing the mechanical properties of Madin-Darby Canine Kidney (MDCK II) cysts.
To investigate how the presence of a luminal space affects the mechanics of epithelial cysts.
To explore tissue-level poroelastic characteristics of epithelial cysts.

Main Methods:

Utilized micropatterning techniques and atomic force microscopy.
Developed a simple in vitro experimental platform for mechanical characterization.
Performed force-relaxation experiments on MDCK II cysts.

Main Results:

Estimated the elasticity of the cyst monolayer, showing significant influence of the luminal space.
Attributed mechanical differences to cell polarization and tissue-level coordination.
Observed tissue-level poroelastic characteristics in cysts, distinct from single cells.

Conclusions:

Provides the first quantitative data on the tissue-level mechanics of well-polarized epithelial cysts.
Offers new insights into the relationship between cyst mechanics and physiology.
Highlights the potential of the developed platform for studying cyst mechanics in health and disease.