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MiR-125b inhibits anaplastic thyroid cancer cell migration and invasion by targeting PIK3CD

Qingao Bu1, Faping You1, Guozheng Pan1

  • 1Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 26, 2017
PubMed

Insights

MicroRNA-125b (miR-125b) suppresses anaplastic thyroid cancer (ATC) cell migration and invasion by targeting phosphoinositide 3-kinase catalytic subunit delta (PIK3CD). This finding reveals miR-125b as a potential therapeutic target for ATC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Dysregulated microRNAs (miRNAs) are involved in cancer development and progression.
  • MicroRNA-125b (miR-125b) acts as a tumor suppressor and is downregulated in various cancers, including thyroid cancer.
  • The specific role and mechanism of miR-125b in anaplastic thyroid cancer (ATC) cell migration and invasion are not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of miR-125b in the migration and invasion of anaplastic thyroid cancer (ATC).
  • To identify the molecular targets of miR-125b in ATC.
  • To explore the therapeutic potential of targeting miR-125b in ATC.

Main Methods:

  • Quantitative real-time PCR to measure miR-125b and phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) expression in ATC tissues and cell lines.
  • Bioinformatic analysis and luciferase reporter assays to confirm PIK3CD as a direct target of miR-125b.
  • In vitro cell migration and invasion assays to assess the functional impact of miR-125b and PIK3CD.
  • Western blot analysis to evaluate the effects of miR-125b on downstream signaling pathways (PI3K, Akt, mTOR).

Main Results:

  • miR-125b was significantly downregulated, while PIK3CD was upregulated in ATC tissues and cell lines.
  • miR-125b directly targeted and downregulated PIK3CD expression in ATC cells.
  • Overexpression of miR-125b inhibited ATC cell migration and invasion, an effect reversed by PIK3CD overexpression.
  • miR-125b suppressed the PI3K/Akt/mTOR signaling pathway in ATC cells.

Conclusions:

  • miR-125b suppresses anaplastic thyroid cancer cell migration and invasion by targeting PIK3CD.
  • The miR-125b/PIK3CD axis represents a potential therapeutic strategy for ATC.
  • Targeting miR-125b may offer novel therapeutic avenues for anaplastic thyroid cancer treatment.
Keywords:
ATCInvasionMigrationPIK3CDmiR-125b

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