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MicroRNA-101 inhibits proliferation, migration and invasion in osteosarcoma cells by targeting ROCK1

Rui Jiang1, Chao Zhang2, Guangyao Liu1

  • 1Department of Orthopedics, China-Japan Union Hospital of Jilin University Changchun 130033, China.

American Journal of Cancer Research
|January 27, 2017
PubMed

Insights

MicroRNA-101 (miR-101) functions as a tumor suppressor in osteosarcoma by targeting ROCK1. Restoring miR-101 inhibits cancer cell growth and metastasis, offering a potential therapeutic strategy for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a prevalent bone cancer in adolescents, characterized by high malignancy and frequent recurrence.
  • The molecular mechanisms underlying osteosarcoma progression, including recurrence and metastasis, require further elucidation.

Purpose of the Study:

  • To investigate the role of microRNA-101 (miR-101) in osteosarcoma.
  • To determine if miR-101 targets Rho-associated coiled-coil containing protein kinase 1 (ROCK1) in osteosarcoma cells.
  • To explore the therapeutic potential of the miR-101/ROCK1 axis in osteosarcoma.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to assess miR-101 expression in patient tissues and cell lines.
  • Dual-luciferase reporter assays confirmed the targeting of ROCK1 3'-UTR by miR-101.
  • Cell viability, migration, invasion, and apoptosis assays were performed following miR-101 or ROCK1 manipulation.
  • Western blotting assessed protein expression levels.
  • Signaling pathway analysis (PI3K/AKT, JAK/STAT) was conducted.

Main Results:

  • miR-101 was significantly downregulated in osteosarcoma tissues and cell lines compared to normal controls.
  • miR-101 directly targeted ROCK1, suppressing its protein expression.
  • Overexpression of miR-101 inhibited osteosarcoma cell proliferation, migration, and invasion, while promoting apoptosis.
  • ROCK1 knockdown mimicked the tumor-suppressive effects of miR-101.
  • miR-101 inhibited tumor growth by downregulating ROCK1, thereby inactivating PI3K/AKT and JAK/STAT signaling pathways.

Conclusions:

  • miR-101 acts as a tumor suppressor in osteosarcoma by targeting ROCK1.
  • The miR-101/ROCK1 pathway represents a promising therapeutic target for osteosarcoma treatment.
  • Modulating miR-101 levels could offer a novel strategy for managing osteosarcoma.
Keywords:
OsteosarcomaROCK1cell apoptosiscell migrationmicroRNA-101

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