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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
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Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae
Si Won Kim1, Seong Won Nho2, Se Pyeong Im1
1Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Journal of Microbiology (Seoul, Korea)
|January 27, 2017
Summary
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) with a new database accurately identified Streptococcus iniae, a fish pathogen, overcoming limitations of existing databases.
Area of Science:
- Aquatic animal health
- Microbiology
- Proteomics
Background:
- Streptococcus iniae causes significant losses in farmed fish, particularly olive flounder.
- Emerging concerns exist regarding S. iniae as a potential zoonotic pathogen.
Purpose of the Study:
- To evaluate the efficacy of MALDI-TOF MS for identifying S. iniae isolates from diseased olive flounder.
- To develop and validate a new S. iniae database for improved identification.
Main Methods:
- Characterization of 89 S. iniae isolates from olive flounder using MALDI-TOF MS.
- Comparison of identification results using the Bruker Daltonics database versus a newly developed laboratory database.
- Proteomic analysis and clustering of isolates using MALDI Biotyper software.
Main Results:
- The Bruker Daltonics database failed to reliably identify S. iniae, often misidentifying isolates as Streptococcus pyogenes.
- The new laboratory database enabled accurate genus-level (100%) and species-level (96.6%) identification of all S. iniae isolates.
- Proteomic analysis revealed distinct clusters within the S. iniae isolates.
Conclusions:
- MALDI-TOF MS, when utilized with an optimized S. iniae database, is a superior method for identifying this fish pathogen.
- This approach offers advantages in speed, cost-efficiency, and accuracy over conventional methods.
- The findings support the use of MALDI-TOF MS for epidemiological and taxonomic studies of S. iniae.
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