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Related Experiment Video

Updated: Mar 8, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

Links between DNA Replication, Stem Cells and Cancer.

Alex Vassilev¹, Melvin L DePamphilis²

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bldg. 6A, Room 3A15, 6 Center Drive, Bethesda, MD 20892-2790, USA. vassilev@mail.nih.gov.

|January 27, 2017

Summary

Cancer arises from DNA replication errors in stem cells or developmental errors involving pluripotent stem cells. Geminin protein is crucial for both, suggesting it as a target for eradicating cancer progenitor cells.

Keywords:

DNA re-replication Geminin cancer stem cells embryonal carcinoma cells embryonic stem cells endoreplication germ cell neoplasia mitotic slippage teratocarcinoma teratoma

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Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

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Published on: May 11, 2016

Area of Science:

Oncology
Developmental Biology
Genetics

Background:

Cancers are broadly classified into age-related types driven by DNA mutations in stem cells and developmental cancers like germ cell neoplasia.
Age-related cancers stem from accumulated mutations during stem cell proliferation, leading to genomic instability and drug resistance.
Germ cell neoplasia originates from misplaced pluripotent stem cells during embryonic development.

Purpose of the Study:

To explore the role of Geminin protein in both major cancer categories.
To identify Geminin as a potential therapeutic target for cancer progenitor cells.

Main Methods:

The study conceptually links DNA replication control in development with cancer mechanisms.
It highlights the dependence of pluripotent stem cells and cancer cells on Geminin.

Main Results:

Geminin protein prevents DNA damage-induced apoptosis triggered by excess DNA replication in both pluripotent stem cells and cancer cells.
A functional link is established between DNA replication control during development and germ cell neoplasia.

Conclusions:

Geminin is essential for preventing DNA damage-induced apoptosis in pluripotent stem cells and cancer cells.
Targeting Geminin offers a potential strategy for eradicating cancer progenitor cells, particularly in germ cell neoplasia.