The midbrain reticular formation as an integration center for the 'near reflex' in the cat

This report describes an experimental study on the localization of converging organization of the near-reflex triad in the chloralose-anesthetized encéphale-isolé cat, in which electromyographic (EMG) recordings were used to elicit responses from the intrinsic and extrinsic eye muscles. Electrical stimulation to several subdivisional areas in the oculomotor nuclear complex evoked EMGs in both the iris sphincter and ciliary muscles. Conduction time from the caudal Edinger-Westphal nucleus to the postganglionic ciliary nerve was about 1.8 ms, whereas that to the iris sphincter muscle was about 6.5 ms. Conduction time from the anteromedian nucleus to the muscle was about 4.5 ms; however, that from the postganglionic short ciliary nerve to the muscle was about 6.7 ms. A direct pathway without synapse in the ciliary ganglion is suggested. Excitatory responses were elicited in the effectors of the near-reflex triad by electrical stimulation of the midbrain reticular formation of the dorsomedial division adjacent to the magnocellular red nucleus (MRFdmMRN). Converging movements in electro-oculography (EOG) were also observed. Conduction time from the MRF to the iris sphincter muscle was about 5.6 ms, whereas that to the postganglionic short ciliary nerve was 5.1 ms. The neural connection between the MRF and the muscle is thought to be mediated by the anteromedian subnucleus. Electrical stimulation of the posteromedial division of the Clare-Bishop (C-B) area evoked a discharge on the MRF and EMGs of all effectors of the triad. The sum of the conduction time from the C-B area to the MRF and that from the MRF to EMGs corresponds well to the latency of EMGs evoked by C-B area stimulation. We conclude that the MRFdmMRN is the supranuclear organization which converges the sensory-motor cortical activities on the precise linkage of the near-reflex triad and becomes an integration center for each nucleus in the oculomotor nuclear complex.