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Updated: Mar 8, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties
J-P Wickhorst1, O Sammra1, A A Hassan2
1Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.
Abstract:
In the present study an Arcanobacterium hippocoleae strain isolated from a uterus swab of an apparently healthy mare could be identified by phenotypic properties, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region and the genes encoding the β subunit of bacterial RNA polymerase (rpoB), elongation factor tu (tuf) and glyceraldehyde 3-phosphate dehydrogenase (gap). The presented data are one of the few reports about the species A. hippocoleae and might help to elucidate the role this species plays in infections of horses.
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