The synthesis of 15N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
Sandro Neuner1, Christoph Kreutz1, Ronald Micura1
1Institute of Organic Chemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck, Austria.
Monatshefte Fur Chemie
|January 28, 2017
Abstract:
We have developed an efficient route for the synthesis of 15N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair interactions in folded RNA using NMR spectroscopic methods.
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