Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 8, 2026

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Published on: July 5, 2024

Transplantation in zebrafish.

J M Gansner¹, M Dang¹, M Ammerman¹

¹Harvard Medical School, Boston, MA, United States.

Methods in Cell Biology

|January 29, 2017

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Limited evidence of elevated microplastics in lower trophic levels downstream of wastewater treatment plants.

Environmental toxicology and chemistry·2026

Same author

Evaluating the impact of sex bias on AI models in musculoskeletal ultrasound of joint recess distension.

PloS one·2025

Same author

Resistance to inflammation underlies enhanced fitness in clonal hematopoiesis.

Science (New York, N.Y.)·2021

Same author

Getting to the Bottom of What's Bugging You: A Curious Case of Delusional Infestation.

Journal of the American Medical Directors Association·2021

Same author

Quality of English inpatient mental health services for people with anxiety or depressive disorders: Findings and recommendations from the core audit of the National Clinical Audit of Anxiety and Depression.

Comprehensive psychiatry·2020

Same author

Chemical screening in zebrafish for novel biological and therapeutic discovery.

Methods in cell biology·2017

Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026

Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026

Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026

Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026

Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026

Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026

See all related articles

Zebrafish transplantation assays enable researchers to track donor cells in vivo. This chapter details methods for cell transplantation in zebrafish embryos and adults for various research applications.

Area of Science:

Developmental Biology
Stem Cell Biology
Regenerative Medicine

Background:

Tissue and cell transplantation are crucial for studying cell development, cell-environment interactions, and stem cell identification.
Distinguishing and tracing donor cells from host cells is essential for transplantation assays.
Zebrafish are an excellent model organism for transplantation due to embryo and adult transparency, aided by pigment mutants.

Purpose of the Study:

To discuss the rationale behind various zebrafish transplantation techniques in embryos and adults.
To provide detailed protocols for four specific transplantation methods.
To highlight the utility of zebrafish in transplantation research.

Main Methods:

Blastula/gastrula transplantation for mosaic analysis.

Keywords:

Blastula CD41:GFP Cancer stem cell Casper Chemical screen Gastrula Hematopoietic stem cell Immunology Melanoma Mosaic analysis Runx1:GFP Stem cell Transplant Tumor Xenotransplantation Zebrafish

More Related Videos

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Published on: May 18, 2011

A Simple and Effective Transplantation Device for Zebrafish Embryos

A Simple and Effective Transplantation Device for Zebrafish Embryos

Published on: August 2, 2021

Related Experiment Videos

Last Updated: Mar 8, 2026

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Published on: July 5, 2024

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Published on: May 18, 2011

A Simple and Effective Transplantation Device for Zebrafish Embryos

A Simple and Effective Transplantation Device for Zebrafish Embryos

Published on: August 2, 2021

Hematopoietic stem cell transplantation.

Chemical screening utilizing a transplantation model.

Tumor transplantation.

Main Results:

Fluorescence-labeled donor cells can be identified and studied in vivo using transgenic techniques and advanced imaging.
Zebrafish enable in vivo tracking of transplanted cells throughout development.
The discussed methods facilitate diverse research applications in developmental and stem cell biology.

Conclusions:

Zebrafish transplantation assays are powerful tools for in vivo cell tracking and developmental studies.
Detailed methods are presented for various applications, including mosaic analysis, stem cell research, chemical screening, and tumor biology.
The transparency and genetic tractability of zebrafish make them ideal for advancing transplantation research.