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Updated: Mar 8, 2026
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
H(O)TA: estimation of DNA methylation and hydroxylation levels and efficiencies from time course data
Charalampos Kyriakopoulos1, Pascal Giehr2, Verena Wolf1
1Computer Science Department, Saarland University, Saarbruecken, Germany.
Motivation:
Methylation and hydroxylation of cytosines to form 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) belong to the most important epigenetic modifications and their vital role in the regulation of gene expression has been widely recognized. Recent experimental techniques allow to infer methylation and hydroxylation levels at CpG dinucleotides but require a sophisticated statistical analysis to achieve accurate estimates.
Results:
We present H(O)TA, a software tool based on a stochastic modeling approach, which simultaneously analyzes time course data from hairpin bisulfite sequencing and hairpin oxidative bisulfite sequencing.
Availability And Implementation:
: https://mosi.uni-saarland.de/HOTA.
Contact:
charalampos.kyriakopoulos@uni-saarland.de or verena.wolf@uni-saarland.de.
