Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.3K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.3K
Protein Complex Assembly02:41

Protein Complex Assembly

17.0K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.0K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.8K
Next-generation Sequencing03:00

Next-generation Sequencing

100.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.0K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

POSERS: A Steganography-Driven Molecular Tagging System Using Randomized DNA Sequences for Secure Authentication.

ACS omega·2025
Same author

In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.

Nucleic acids research·2025
Same author

Priming thermotolerance: unlocking heat resilience for climate-smart crops.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2025
Same author

Stress resilience in plants: the complex interplay between heat stress memory and resetting.

The New phytologist·2025
Same author

Mining genomic regions associated with agronomic and biochemical traits in quinoa through GWAS.

Scientific reports·2024
Same author

An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.

ACS synthetic biology·2024
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Mar 8, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.9K

AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies.

Lena Hochrein1, Fabian Machens1, Juergen Gremmels2

  • 1University of Potsdam, Cell2Fab Research Unit, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany.

Nucleic Acids Research
|January 29, 2017
PubMed
Summary
This summary is machine-generated.

AssemblX is a new, user-friendly strategy for assembling large DNA constructs. This software-assisted method enables rapid design, building, and testing of complex genetic circuits for synthetic biology applications.

More Related Videos

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.1K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.7K

Related Experiment Videos

Last Updated: Mar 8, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.9K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.1K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.7K

Area of Science:

  • Synthetic biology
  • Molecular biology
  • Genetic engineering

Background:

  • Assembling large DNA constructs for entire pathways is a significant challenge in synthetic biology.
  • Current methods often require extensive parts domestication and can be time-consuming.

Purpose of the Study:

  • To present AssemblX, a novel, user-friendly, and highly efficient multi-gene assembly strategy.
  • To enable rapid design, construction, and testing of complex DNA constructs with numerous functional units.

Main Methods:

  • Software-assisted, scar-free, overlap-based, and sequence-independent gene assembly.
  • Standardized, polymerase chain reaction-free, and sequence-independent multi-gene module assembly using rare cutting restriction enzymes and optimized adapters.
  • Selection and marker switching strategies for reliability and effectiveness.

Main Results:

  • AssemblX facilitates the assembly of DNA constructs with up to 25 functional units from over 75 subunits.
  • The method allows unrestricted transcriptional unit design without parts domestication.
  • The assembly product is easily transferable to various expression hosts.

Conclusions:

  • AssemblX offers a reliable, rapid, and effective solution for constructing large DNA constructs.
  • The strategy is suitable for researchers across various fields, including synthetic biology, molecular biology, and genetic engineering.
  • This approach simplifies the process of building complex genetic circuits.