Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

565
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
565
Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
Sanger Sequencing01:57

Sanger Sequencing

773.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.0K
Next-generation Sequencing03:00

Next-generation Sequencing

97.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.6K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.

Nucleic acids research·2025
Same author

An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.

ACS synthetic biology·2024
Same author

PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in <i>Saccharomyces cerevisiae</i>.

ACS synthetic biology·2023
Same journal

Deciphering Physicochemical Properties of Urinary Proteins from Stone Formers as Macromolecular Prolithogenic Factors to Drive Calcium Oxalate Stone Development.

ACS omega·2026
Same journal

Impact of an Artificial Albumin Corona on Surface Charge-Driven Nano-Bio Interactions and Cytotoxicity of Silver Nanoparticles.

ACS omega·2026
Same journal

Structural and Functional Disruption of Thiopurine S‑Methyltransferase by the A80P Variant: A Simulation and Genotyping Study.

ACS omega·2026
Same journal

CRISPR/Cas12a2-Mediated Ultrasensitive Assay for Rapid Detection of H1N1 Influenza Virus RNA.

ACS omega·2026
Same journal

Photocatalytic Treatment of Real Sugar Industry Wastewater Using Lignocellulosic Biomass-Derived Hydrochar/g-CN.

ACS omega·2026
Same journal

Electrochemical Dopamine Biosensor Based on Plant-Derived Peroxidase Immobilized on Titanate Nanowires.

ACS omega·2026
See all related articles

Related Experiment Video

Updated: Jan 11, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K

POSERS: A Steganography-Driven Molecular Tagging System Using Randomized DNA Sequences for Secure Authentication.

Ali Tafazoli Yazdi1, Peter Nejjar2, Lena Hochrein1

  • 1University of Potsdam, Institute of Biochemistry and Biology, Faculty of Science, Potsdam 14476, Germany.

ACS Omega
|November 10, 2025
PubMed
Summary
This summary is machine-generated.

Counterfeiting is a major threat. A new DNA tagging system, POSERS (Position-Oriented Scattering of Elements among a Randomized Sequence), uses randomized DNA libraries for robust, scalable anti-counterfeiting solutions.

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K
DNA Stable-Isotope Probing DNA-SIP
14:57

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

45.0K

Related Experiment Videos

Last Updated: Jan 11, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K
DNA Stable-Isotope Probing DNA-SIP
14:57

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

45.0K

Area of Science:

  • Molecular biology
  • Materials science
  • Cryptography

Background:

  • Counterfeiting poses significant financial and health risks across industries.
  • Existing DNA tagging methods are vulnerable to replication due to fixed sequences and advancing technologies.
  • There is a need for advanced, secure, and scalable anti-counterfeiting solutions.

Purpose of the Study:

  • To introduce POSERS (Position-Oriented Scattering of Elements among a Randomized Sequence), a novel steganographic DNA tagging system.
  • To develop a customizable and replicable-resistant anti-counterfeiting strategy.
  • To provide a scalable and future-proof solution for high-risk products.

Main Methods:

  • POSERS encodes product-specific constraints into diverse, randomized DNA libraries.
  • DNA libraries are generated in a single synthesis step for cost-effective, large-scale deployment.
  • Security is validated mathematically and experimentally using next-generation sequencing and authentication tests.

Main Results:

  • POSERS demonstrated robustness against replication attempts, including PCR-based copying and reverse-engineering.
  • The system successfully distinguished genuine tags from forgeries under conservative security assumptions.
  • Unique DNA libraries were generated for each product in a batch, enabling cost-effective deployment.

Conclusions:

  • POSERS establishes a new standard for secure molecular tagging, offering customizable design and resilience.
  • The system is adaptable to various molecular carriers, with DNA being a stable and cost-effective option.
  • POSERS provides a scalable, future-proof solution against current and emerging counterfeiting threats.