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Related Experiment Videos

Internal cell manipulation using infrared laser traps.

A Ashkin1, J M Dziedzic

  • 1Laser Science Research Department, AT&L Bell Laboratories, Holmdel, NJ 07733-1988.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1989
PubMed
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Infrared laser traps reveal plant cell cytoplasm

Area of Science:

  • Cell Biology
  • Biophysics
  • Plant Science

Background:

  • Understanding the mechanical properties of cytoplasm is crucial for cell function.
  • Previous methods for studying rheology often perturb living cells.

Purpose of the Study:

  • To investigate the rheological properties of plant cell cytoplasm.
  • To demonstrate the utility of infrared laser traps for cell manipulation and analysis.

Main Methods:

  • Utilized infrared laser traps to apply controlled forces within plant cells.
  • Plucked long filaments of cytoplasm to analyze their mechanical behavior.
  • Performed internal cell surgery to reposition organelles like chloroplasts and nuclei.

Main Results:

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  • Cytoplasmic filaments exhibited viscoelastic properties including plastic flow, necking, and stress relaxation.
  • Infrared laser traps enabled probing of local rheological properties in unperturbed cells.
  • Demonstrated successful gross manipulation of organelles within living cells.
  • Conclusions:

    • Infrared laser trapping offers a novel method for studying cellular mechanics.
    • The technique allows for precise investigation of cytoplasmic rheology and organelle dynamics.
    • This approach has implications for understanding cytoplasmic streaming and organelle attachment.